Logo

S2C2 CryoEM Modeling workshop - Shared screen with speaker view
Chris Colbert
23:57
Yes and yes
Chris Colbert
49:24
Left
Joana Paulino
52:22
can someone repeat the question?
gkaur1
56:34
Introduce noise
Joana Paulino
01:03:29
I do
iswaryapj
01:16:50
can you increase the microphone volume, so that I can clearly hear the audience.. there is as lot of discussion...
iswaryapj
01:17:04
ok
iswaryapj
01:17:08
fine
Joana Paulino
01:17:25
when the maps are deposited on EMDB is all the information regarding sharpening and other processing protocols added with it?
Chris Colbert
01:25:30
Right
Chris Colbert
01:25:49
Molecular boundary
gkaur1
01:31:15
please use mouse pointer if possible
iswaryapj
02:13:44
ok
bdonohoe
02:29:07
Audio it good
gkaur1
02:29:17
yes
Joana Paulino
02:42:22
in maps where the resolution and information is very heterogeneous through out the map could this rescaling process be negatively influencing some regions of the map more than others?
gkaur1
03:01:09
Should we look for water molecules in maps <5 Angstroms?
gkaur1
03:03:23
3-5 angstrom
Michael Schmid
03:36:10
any questions? I lost the chat thread for a bit
Chris Colbert
03:37:29
How does real_space refinement weight the data? Like the X-ray term during X-ray refinement allows you to overcome stereo chemistry and protein chemistry at hi-res?
Chris Colbert
03:41:47
Does it vary over regions of the structure since CryoEM structures can have variable resolutions over the whole structure?
Michael Schmid
04:07:34
We will reconvene at 1:40 PST.
Joana Paulino
05:25:00
could you repeat the name of the map you loaded. I am getting an error that the .map file I load is not an real_map file. I am sorry to interrupt.
gkaur1
05:35:41
I get an error: the model contains errors which are not in the scattering table “electron” unknown atom types: 01
gkaur1
05:36:56
i downloaded the pub file from rocs and extracted the chain a
gkaur1
05:37:03
rcsb
gkaur1
05:37:24
Ok. Thank you.
Chris Colbert
05:57:04
Are cores the same as processors on Macs?
Chris Colbert
05:59:43
Will PHENIX ignore you if you select the wrong number?
Chris Colbert
06:00:50
Ha ha ha