Yes and yes

Left

can someone repeat the question?

Introduce noise

I do

can you increase the microphone volume, so that I can clearly hear the audience.. there is as lot of discussion...

ok

fine

when the maps are deposited on EMDB is all the information regarding sharpening and other processing protocols added with it?

Right

Molecular boundary

please use mouse pointer if possible

ok

Audio it good

yes

in maps where the resolution and information is very heterogeneous through out the map could this rescaling process be negatively influencing some regions of the map more than others?

Should we look for water molecules in maps <5 Angstroms?

3-5 angstrom

any questions? I lost the chat thread for a bit

How does real_space refinement weight the data? Like the X-ray term during X-ray refinement allows you to overcome stereo chemistry and protein chemistry at hi-res?

Does it vary over regions of the structure since CryoEM structures can have variable resolutions over the whole structure?

We will reconvene at 1:40 PST.

could you repeat the name of the map you loaded. I am getting an error that the .map file I load is not an real_map file. I am sorry to interrupt.

I get an error: the model contains errors which are not in the scattering table “electron” unknown atom types: 01

i downloaded the pub file from rocs and extracted the chain a

rcsb

Ok. Thank you.

Are cores the same as processors on Macs?

Will PHENIX ignore you if you select the wrong number?

Ha ha ha